3UCG

Crystal structure of a RNA binding domain of polyadenylate-binding protein (PABPN1) from Homo sapiens at 1.95 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-21
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97925,0.97894
Spacegroup name	I 2 3
Unit cell lengths	92.678, 92.678, 92.678
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.307 - 1.950
R-factor	0.165
Rwork	0.163
R-free	0.20960
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.714
Data reduction software	XDS
Data scaling software	XSCALE (December 6, 2010)
Phasing software	SHELX
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.307		2.020
High resolution limit [Å]	1.950	4.190	1.950
Rmerge	0.045	0.019	0.771
Number of reflections	9771	1881	1820
<I/σ(I)>	18.08	55.3	1.8
Completeness [%]	99.2	98.9	97.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	2.0M (NH4)2SO4, 2.0% PEG-400, 0.1M HEPES pH 7.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K