3UC9
Crystal Structure of Yeast Irc6p - A Novel Type of Conserved Clathrin Accessory Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 65 |
Unit cell lengths | 61.638, 61.638, 95.144 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.270 - 1.800 |
R-factor | 0.1977 |
Rwork | 0.197 |
R-free | 0.21810 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.104 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXD |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 80.000 | 80.000 | 1.860 |
High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
Rmerge | 0.066 | 0.023 | 0.541 |
Number of reflections | 18511 | ||
<I/σ(I)> | 13.1 | 45.27 | 3.96 |
Completeness [%] | 97.4 | 96.3 | 96.5 |
Redundancy | 6.9 | 4.8 | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2 M ammonium sulfate, 0.1 MES, 30 % w/v PEG monomethyl ether 5000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |