3UC2
Crystal structure of a DUF4426 family protein (PA0388) from Pseudomonas aeruginosa PAO1 at 2.09 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-21 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.91837,0.97916,0.97886 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.453, 45.436, 294.498 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.083 - 2.090 |
R-factor | 0.2122 |
Rwork | 0.211 |
R-free | 0.23400 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.110 |
Data reduction software | XDS |
Data scaling software | XSCALE (December 6, 2010) |
Phasing software | SHELX |
Refinement software | BUSTER-TNT (2.10.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.083 | 49.080 | 2.160 |
High resolution limit [Å] | 2.090 | 4.490 | 2.090 |
Rmerge | 0.048 | 0.024 | 0.619 |
Number of reflections | 36206 | 3788 | 3377 |
<I/σ(I)> | 16.61 | 45 | 2.2 |
Completeness [%] | 98.6 | 94.5 | 99.8 |
Redundancy | 4 | 3.99 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 277 | 0.17M (NH4)2SO4, 15.0% Glycerol, 25.5% PEG-4000, No Buffer pH 4.2, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |