3UBO
The crystal structure of adenosine kinase from Sinorhizobium meliloti
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 156.307, 156.307, 89.081 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.122 - 2.601 |
R-factor | 0.237 |
Rwork | 0.235 |
R-free | 0.26740 |
Structure solution method | SAD |
RMSD bond length | 0.022 |
RMSD bond angle | 2.099 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.074 | 0.550 |
Number of reflections | 38782 | |
<I/σ(I)> | 2.9 | 2.3 |
Completeness [%] | 97.0 | 100 |
Redundancy | 11.2 | 11 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 1.1 M Ammonium tartrate dibasic pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |