3UB7
Periplasmic portion of the Helicobacter pylori chemoreceptor TlpB with acetamide bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 79.587, 81.986, 94.137 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.820 - 1.400 |
R-factor | 0.1392 |
Rwork | 0.138 |
R-free | 0.16230 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.484 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.430 |
High resolution limit [Å] | 1.400 | 3.450 | 1.400 |
Rmerge | 0.044 | 0.028 | 0.517 |
Number of reflections | 107983 | ||
<I/σ(I)> | 13.2 | ||
Completeness [%] | 88.7 | 81 | 89.3 |
Redundancy | 4 | 4.2 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.1 M PIPES pH 6.5, 1.9 M ammonium sulfate, vapor diffusion, hanging drop, temperature 277K |