3UB6
Periplasmic portion of the Helicobacter pylori chemoreceptor TlpB with urea bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.608, 81.881, 94.534 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.270 - 1.380 |
| R-factor | 0.1245 |
| Rwork | 0.123 |
| R-free | 0.14640 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.484 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.400 |
| High resolution limit [Å] | 1.380 | 3.750 | 1.380 |
| Rmerge | 0.071 | 0.051 | 0.490 |
| Number of reflections | 126318 | ||
| <I/σ(I)> | 13.3 | ||
| Completeness [%] | 99.4 | 99.9 | 98.3 |
| Redundancy | 8.6 | 6.5 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.1 M PIPES pH 6.5, 22% PEG 3350, 0.2 M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
