3UB5
Profilin:actin with a wide open nucleotide cleft
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-06-11 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.000, 72.000, 186.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.240 - 2.200 |
| R-factor | 0.21928 |
| Rwork | 0.215 |
| R-free | 0.28977 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.874 |
| Data reduction software | EVAL15 |
| Data scaling software | SADABS |
| Refinement software | REFMAC (5.6.0119) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.400 | |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 26998 | |
| Completeness [%] | 99.1 | 99.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 277 | 1.3M potassium phosphate pH 7.3, 0.5M ATP, 0.2M CaCl2, 1mM DTT, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
