3UB1
Ntf2 like protein involved in plasmid conjugation
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-21 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91837, 0.97860, 0.98133 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 121.495, 121.842, 125.526 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.760 - 1.800 |
| R-factor | 0.165 |
| Rwork | 0.163 |
| R-free | 0.19800 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.327 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.890 | |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.480 | |
| Number of reflections | 172185 | |
| <I/σ(I)> | 5.2 | |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100MM BIS-TRIS, 50MM CACL2, 28-30%(V/ V) PEG MME 550, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
