3UAU
Crystal structure of the lipoprotein JlpA
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97943 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 111.632, 111.632, 170.369 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.900 - 2.700 |
R-factor | 0.24315 |
Rwork | 0.242 |
R-free | 0.27221 |
Structure solution method | MAD |
RMSD bond length | 0.019 |
RMSD bond angle | 1.669 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.066 | 0.339 |
Number of reflections | 27364 | |
Completeness [%] | 90.5 | 76 |
Redundancy | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 288 | 32-36% MPD, 20-30 mM calcium chloride, 0.1 M sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |