3UAT
Guanylate Kinase Domains of the MAGUK Family Scaffold Proteins as Specific Phospho-Protein Binding Modules
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Detector technology | CCD |
| Collection date | 2011-06-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9817 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.566, 66.155, 55.155 |
| Unit cell angles | 90.00, 102.44, 90.00 |
Refinement procedure
| Resolution | 46.449 - 2.700 |
| R-factor | 0.1896 |
| Rwork | 0.187 |
| R-free | 0.24150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.765 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.700 |
| Number of reflections | 9281 |
| Completeness [%] | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.8 | 289 | 0.2M Sodium nitrate, 20% w/v Polyethylene glycol 3350 , pH 6.8, EVAPORATION, temperature 289K |
