3UAP
Crystal structure of glutathione transferase (TARGET EFI-501774) from methylococcus capsulatus str. bath
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-07 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 129.246, 129.246, 129.246 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.080 - 2.800 |
| R-factor | 0.19464 |
| Rwork | 0.193 |
| R-free | 0.25921 |
| Structure solution method | MR |
| Starting model (for MR) | 2dsa |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.050 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 10645 | |
| <I/σ(I)> | 8.9 | 0.8 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 11.9 | 11.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.2M SODIUM CHLORIDE, 0.1M TRIS-HCL, 30% PEG3000, PH 7.0, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 294K |
