3UAH
Structure of the Shq1 specific domain from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-26 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.087, 50.126, 53.951 |
| Unit cell angles | 102.19, 93.75, 97.97 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| R-factor | 0.16972 |
| Rwork | 0.168 |
| R-free | 0.19826 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.212 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.057 | 0.132 |
| Number of reflections | 50317 | |
| <I/σ(I)> | 42.5 | 14.1 |
| Completeness [%] | 96.8 | 95.3 |
| Redundancy | 3.9 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1M tri-sodium citrate, 0.2M ammonium acetate, 20%(w/v) PEG 4000, 9% isopropanol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
