3U9Z
Crystal structure between actin and a protein construct containing the first beta-thymosin domain of drosophila ciboulot (residues 2-58) with the three mutations N26D/Q27K/D28S
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9300 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.795, 75.181, 117.708 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.000 - 2.090 |
R-factor | 0.1647 |
Rwork | 0.160 |
R-free | 0.22660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1sqk |
RMSD bond length | 0.020 |
RMSD bond angle | 1.452 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (1.3.2) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.000 | 2.100 | |
High resolution limit [Å] | 2.090 | 12.500 | 2.090 |
Rmerge | 0.092 | 0.027 | 0.475 |
Number of reflections | 24772 | 143 | 346 |
<I/σ(I)> | 26.84 | 47.13 | 7.04 |
Completeness [%] | 99.9 | 97.3 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 6.5 | 298 | 18% PEG3350, 0.05M NaAcetate pH4.7, 0.1M MgAcetate pH6.5, 0.32M Guanidine HCl, 0.8% Dioxane, hanging drop, temperature 298K |