3U90
apoferritin: complex with SDS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-10 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.000 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 181.830, 181.830, 181.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.840 - 1.900 |
| R-factor | 0.187 |
| Rwork | 0.185 |
| R-free | 0.20770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xz1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.897 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.840 | 1.960 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.088 | 0.387 |
| Number of reflections | 21314 | |
| <I/σ(I)> | 43.4 | 9 |
| Completeness [%] | 98.6 | 87.6 |
| Redundancy | 34.09 | 34.09 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.05 M cadmium sulfate, 0.1 M HEPES, 1.0 M sodium acetate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
