3U8Z
human merlin FERM domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-16 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.999990 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 105.446, 105.446, 329.997 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.360 - 2.640 |
R-factor | 0.2017 |
Rwork | 0.200 |
R-free | 0.22940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1h4r |
RMSD bond length | 0.008 |
RMSD bond angle | 0.870 |
Data reduction software | XDS |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | BUSTER (2.13.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 105.450 | 2.780 |
High resolution limit [Å] | 2.640 | 2.640 |
Rmerge | 0.063 | 0.377 |
Number of reflections | 52570 | |
<I/σ(I)> | 19.9 | 1.9 |
Completeness [%] | 95.2 | 79.7 |
Redundancy | 7.2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | protein in 50 mM Tris, pH 8.0, 300 mM sodium chloride, reservoir: 4.5% PEG4000, 0.2 M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 277K |