3U8C
Crystal structure of monomeric reversibly photoswitchable red fluorescent protein rsTagRFP in the ON state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.564, 69.336, 73.699 |
| Unit cell angles | 78.69, 83.60, 71.97 |
Refinement procedure
| Resolution | 26.887 - 2.188 |
| R-factor | 0.1628 |
| Rwork | 0.160 |
| R-free | 0.24410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.957 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.280 |
| High resolution limit [Å] | 2.188 | 4.730 | 2.188 |
| Rmerge | 0.048 | 0.027 | 0.338 |
| Number of reflections | 45928 | ||
| <I/σ(I)> | 9.6 | ||
| Completeness [%] | 98.1 | 98.9 | 96.8 |
| Redundancy | 2 | 2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.2 M Ammonium Citrate, 20% PEG 3350, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
