3U7B
A new crystal structure of a Fusarium oxysporum GH10 xylanase reveals the presence of an extended loop on top of the catalytic cleft
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-20 |
Detector | MAR CCD 165 mm |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 124.220, 124.220, 284.040 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 17.910 - 1.940 |
R-factor | 0.2142 |
Rwork | 0.213 |
R-free | 0.24183 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3cui |
RMSD bond length | 0.008 |
RMSD bond angle | 1.069 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | BALBES |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 17.900 |
High resolution limit [Å] | 1.900 |
Number of reflections | 173419 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 17% PEG 10000, 0.1 M ammonium acetate, 0.1 M Bis-Tris, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |