3U6U
Crystal structure of the putative acetylglutamate kinase from thermus thermophilus
Replaces: 2EGXExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL32B2 |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 2006-12-25 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97937 |
| Spacegroup name | P 65 |
| Unit cell lengths | 155.601, 155.601, 79.535 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.900 - 1.920 |
| R-factor | 0.225 |
| Rwork | 0.222 |
| R-free | 0.23700 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.990 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.075 | 0.601 |
| Number of reflections | 163557 | |
| Completeness [%] | 95.4 | 58.2 |
| Redundancy | 9.5 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.5M AMMONIUM SULFATE, 0.1M TRI SODIUM CITRATE DEHYDRATE, 1.0M LITHIUM SULFATE MONOHYDRATE, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
