3U6N
Open Structure of the BK channel Gating Ring
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 137.648, 210.816, 238.758 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.220 - 3.610 |
| R-factor | 0.261 |
| Rwork | 0.260 |
| R-free | 0.28920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.088 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.730 |
| High resolution limit [Å] | 3.600 | 7.750 | 3.600 |
| Rmerge | 0.103 | 0.037 | 0.794 |
| Number of reflections | 80016 | ||
| <I/σ(I)> | 10 | ||
| Completeness [%] | 99.4 | 97.5 | 99.5 |
| Redundancy | 4.2 | 4 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 293 | PEG 4000, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
