3U6N
Open Structure of the BK channel Gating Ring
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-04 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.075 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 137.648, 210.816, 238.758 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.220 - 3.610 |
R-factor | 0.261 |
Rwork | 0.260 |
R-free | 0.28920 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.088 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.730 |
High resolution limit [Å] | 3.600 | 7.750 | 3.600 |
Rmerge | 0.103 | 0.037 | 0.794 |
Number of reflections | 80016 | ||
<I/σ(I)> | 10 | ||
Completeness [%] | 99.4 | 97.5 | 99.5 |
Redundancy | 4.2 | 4 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 293 | PEG 4000, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |