3U6L
MutM set 2 CpGo
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-28 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.188, 93.541, 104.650 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.690 - 1.970 |
R-factor | 0.18 |
Rwork | 0.179 |
R-free | 0.20700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.867 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.690 | 2.000 |
High resolution limit [Å] | 1.970 | 1.970 |
Number of reflections | 32218 | |
<I/σ(I)> | 4.7 | |
Redundancy | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 298 | PEG 8K, sodium cacodylate, glycerol, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |