3U5N
Crystal structure of the complex of TRIM33 PHD-Bromo and H3(1-20)K9me3K14ac histone peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 197 |
Detector technology | CCD |
Collection date | 2011-03-08 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.28215 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 97.586, 113.737, 39.861 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.792 - 1.950 |
R-factor | 0.2051 |
Rwork | 0.202 |
R-free | 0.25610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3u5m |
RMSD bond length | 0.008 |
RMSD bond angle | 1.083 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 32561 | |
<I/σ(I)> | 24.4 | 2.5 |
Completeness [%] | 98.0 | 99.4 |
Redundancy | 4.3 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M ammonium tartrate and 20% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |