3U58
Crystal Structure of the Tetrahymena telomerase processivity factor Teb1 AB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97944 |
| Spacegroup name | P 1 |
| Unit cell lengths | 83.046, 83.110, 82.885 |
| Unit cell angles | 108.45, 111.59, 108.42 |
Refinement procedure
| Resolution | 33.580 - 2.613 |
| R-factor | 0.2232 |
| Rwork | 0.219 |
| R-free | 0.25870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.134 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 5.600 | 2.600 |
| Rmerge | 0.103 | ||
| Number of reflections | 48660 | ||
| <I/σ(I)> | 21.366 | ||
| Completeness [%] | 94.7 | ||
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 277 | 10% PEG6000, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
