3U56
Crystal structure of mutant ribosomal protein T217V TthL1 in complex with 80nt 23S RNA from Thermus thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 2011-01-18 |
Detector | MAR scanner 345 mm plate |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.690, 75.410, 85.880 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.300 - 2.100 |
R-factor | 0.194 |
Rwork | 0.191 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ad2 1mzp |
RMSD bond length | 0.006 |
RMSD bond angle | 0.935 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.300 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.099 | 0.408 |
Number of reflections | 28142 | |
<I/σ(I)> | 12.57 | 3.38 |
Completeness [%] | 98.5 | 90 |
Redundancy | 4.6 | 4.01 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.6 | 295 | 50mM NaCl, 2.5M Ammonium sulfate, 50mM MES, 10mM Magnesium acetate , pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |