3U50

Crystal Structure of the Tetrahymena telomerase processivity factor Teb1 OB-C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-06-10
Detector	MAR scanner 300 mm plate
Spacegroup name	P 31 2 1
Unit cell lengths	80.609, 80.609, 82.081
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.180 - 2.500
R-factor	0.218
Rwork	0.214
R-free	0.25300
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.139
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.7_650)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.058	0.357
Number of reflections	10822
<I/σ(I)>	17.2
Completeness [%]	97.9	82.2
Redundancy	10	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.2	277	3.8 M NaCl, pH 5.2, vapor diffusion, sitting drop, temperature 277K