3U50
Crystal Structure of the Tetrahymena telomerase processivity factor Teb1 OB-C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-06-10 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 80.609, 80.609, 82.081 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.180 - 2.500 |
| R-factor | 0.218 |
| Rwork | 0.214 |
| R-free | 0.25300 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.139 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.058 | 0.357 |
| Number of reflections | 10822 | |
| <I/σ(I)> | 17.2 | |
| Completeness [%] | 97.9 | 82.2 |
| Redundancy | 10 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.2 | 277 | 3.8 M NaCl, pH 5.2, vapor diffusion, sitting drop, temperature 277K |
