3U50
Crystal Structure of the Tetrahymena telomerase processivity factor Teb1 OB-C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-06-10 |
Detector | MAR scanner 300 mm plate |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 80.609, 80.609, 82.081 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.180 - 2.500 |
R-factor | 0.218 |
Rwork | 0.214 |
R-free | 0.25300 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.139 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | PHENIX (1.7_650) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.058 | 0.357 |
Number of reflections | 10822 | |
<I/σ(I)> | 17.2 | |
Completeness [%] | 97.9 | 82.2 |
Redundancy | 10 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.2 | 277 | 3.8 M NaCl, pH 5.2, vapor diffusion, sitting drop, temperature 277K |