3U4Z
Crystal Structure of the Tetrahymena telomerase processivity factor Teb1 OB-B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-02 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.551, 65.841, 57.845 |
| Unit cell angles | 90.00, 100.01, 90.00 |
Refinement procedure
| Resolution | 28.592 - 2.300 |
| R-factor | 0.2249 |
| Rwork | 0.220 |
| R-free | 0.26630 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.990 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 6.240 | 2.300 |
| Rmerge | 0.073 | 0.033 | 0.262 |
| Number of reflections | 10731 | ||
| <I/σ(I)> | 11 | ||
| Completeness [%] | 93.5 | 96.5 | 74.5 |
| Redundancy | 3.5 | 3.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 3.6 M Na-Formate, vapor diffusion, sitting drop, temperature 277K |
