3U49
Crystal structure of YwfH, NADPH dependent reductase involved in Bacilysin biosynthesis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-12-24 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.850, 136.990, 66.700 |
Unit cell angles | 90.00, 117.99, 90.00 |
Refinement procedure
Resolution | 68.500 - 1.750 |
R-factor | 0.19044 |
Rwork | 0.189 |
R-free | 0.22180 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.999 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.500 | 1.840 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.089 | 0.487 |
Number of reflections | 102319 | |
<I/σ(I)> | 13.4 | 4.1 |
Completeness [%] | 99.1 | 100 |
Redundancy | 7.6 | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7.5 | 293 | 0.1M HEPES, 1.4M Tri-sodium citrate dihydrate, pH 7.5, Microbatch, temperature 293K |