3U48
From soil to structure: a novel dimeric family 3-beta-glucosidase isolated from compost using metagenomic analysis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97740 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 121.488, 121.488, 242.859 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.593 - 2.200 |
R-factor | 0.169 |
Rwork | 0.169 |
R-free | 0.21890 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.957 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.117 | 0.612 |
Number of reflections | 88156 | |
<I/σ(I)> | 15.4 | 1.73 |
Completeness [%] | 95.0 | 65.3 |
Redundancy | 10.4 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1 M sodium citrate and 30% (w/v) PEG 3350, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |