3U3Y
Mouse TREX1 D200H mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Detector technology | CCD |
| Collection date | 2008-10-06 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.066, 57.861, 68.227 |
| Unit cell angles | 90.00, 108.11, 90.00 |
Refinement procedure
| Resolution | 22.724 - 2.280 |
| R-factor | 0.2162 |
| Rwork | 0.213 |
| R-free | 0.27530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.096 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 2.280 |
| Rmerge | 0.072 |
| Number of reflections | 21089 |
| <I/σ(I)> | 8.8 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 298 | 16% PEG 4000, 0.1 M MES pH 6.0, 2% 1,4-butanediol, VAPOR DIFFUSION, temperature 298K |
