3U3Q

The S-SAD phased crystal structure of the ecto-domain of Death Receptor 6 (DR6)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-11
Detector	ADSC QUANTUM 315
Wavelength(s)	2.000
Spacegroup name	P 61 2 2
Unit cell lengths	77.946, 77.946, 186.526
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.700
R-factor	0.19689
Rwork	0.195
R-free	0.23544
Structure solution method	SAD
RMSD bond length	0.020
RMSD bond angle	2.134
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.700
Number of reflections	9338
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.2M ammonium acetate, 0.1M sodium citrate tribasic dehydrate (pH5.0-6.0), 25-30% PEG 4000 , VAPOR DIFFUSION, HANGING DROP, temperature 289K