3U3I

A RNA binding protein from Crimean-Congo hemorrhagic fever virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9798
Spacegroup name	P 21 21 21
Unit cell lengths	58.339, 67.898, 131.506
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.639 - 2.304
R-factor	0.2256
Rwork	0.224
R-free	0.25720
Structure solution method	SAD
RMSD bond length	0.025
RMSD bond angle	2.461
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.6.1_357)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300
Number of reflections	22556
Completeness [%]	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	291	KCl, 20%(w/v) PEG3350, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K