3U37
An Acetyl Xylan Esterase (Est2A) from the Rumen Bacterium Butyrivibrio proteoclasticus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 |
| Unit cell lengths | 96.128, 90.282, 99.175 |
| Unit cell angles | 99.80, 90.21, 92.56 |
Refinement procedure
| Resolution | 24.856 - 2.100 |
| R-factor | 0.208 |
| Rwork | 0.206 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.170 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 24.856 |
| High resolution limit [Å] | 2.100 |
| Number of reflections | 171075 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 291 | 8% (w/v) PEG 4000, 0.1 M Sodium acetate pH 4.6. , VAPOR DIFFUSION, HANGING DROP, temperature 291K |
