3U2W
Crystal Structure of Human Glycogenin-1 (GYG1) complexed with manganese and glucose or a glucal species
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-05-24 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.290, 80.180, 69.530 |
| Unit cell angles | 90.00, 100.67, 90.00 |
Refinement procedure
| Resolution | 22.585 - 1.680 |
| R-factor | 0.15876 |
| Rwork | 0.157 |
| R-free | 0.19041 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q4s |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.499 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.780 | 1.770 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.055 | 0.546 |
| Number of reflections | 58070 | |
| <I/σ(I)> | 13.9 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293.15 | 25% PEG 3350, 0.2M Li2(SO4), 0.1M Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |
