3U2T
Crystal Structure of Human Glycogenin-1 (GYG1) complexed with manganese
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-10-22 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 58.350, 101.060, 47.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.990 - 2.050 |
| R-factor | 0.20144 |
| Rwork | 0.199 |
| R-free | 0.25114 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q4s |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.521 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.200 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.104 | 0.652 |
| Number of reflections | 18330 | |
| <I/σ(I)> | 10 | 2.1 |
| Completeness [%] | 99.7 | 99.2 |
| Redundancy | 5 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 22.5% PEG smears, 0.2M Li2(SO4), 0.05M ZnCl2, 0.1M Bis-Tris, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |
