3U2H
Crystal structure of the C-terminal DUF1608 domain of the Methanosarcina acetivorans S-layer (MA0829) protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 74.675, 49.582, 84.952 |
| Unit cell angles | 90.00, 106.15, 90.00 |
Refinement procedure
| Resolution | 81.600 - 2.360 |
| R-factor | 0.2108 |
| Rwork | 0.209 |
| R-free | 0.25330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3u2g |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.174 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 90.000 | 90.000 | 2.440 |
| High resolution limit [Å] | 2.360 | 5.080 | 2.360 |
| Rmerge | 0.064 | 0.392 | |
| Number of reflections | 12345 | ||
| <I/σ(I)> | 12.3 | 21.6 | 6.8 |
| Completeness [%] | 99.7 | 98.8 | 100 |
| Redundancy | 6.8 | 6.5 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 0.2M sodium sulfate, 20% PEG3350, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
