3U24
The structure of a putative lipoprotein of unknown function from Shewanella oneidensis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-24 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 96.427, 96.427, 133.947 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.370 - 2.250 |
| R-factor | 0.16409 |
| Rwork | 0.162 |
| R-free | 0.20045 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.494 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.290 |
| High resolution limit [Å] | 2.250 | 6.100 | 2.250 |
| Rmerge | 0.101 | 0.068 | 0.474 |
| Number of reflections | 34837 | ||
| <I/σ(I)> | 9.2 | ||
| Completeness [%] | 99.9 | 99.3 | 100 |
| Redundancy | 9.2 | 8.7 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 297 | 0.2M zinc acetate, 0.1M imidazole pH 8, 20% PEG 3000, 10% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
