3U1U

Crystal structure of RNA polymerase-associated protein RTF1 homolog Plus-3 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Detector technology	IMAGE PLATE
Collection date	2011-08-17
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	47.479, 50.369, 69.776
Unit cell angles	90.00, 103.37, 90.00

Refinement procedure

Resolution	28.540 - 1.800
R-factor	0.2036
Rwork	0.202
R-free	0.23490
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.355
Data reduction software	XDS
Data scaling software	SCALA (CCP4_3.3.16)
Phasing software	SHELX
Refinement software	REFMAC (refmac_5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.900
High resolution limit [Å]	1.800	5.690	1.800
Rmerge	0.076	0.020	0.890
Total number of observations		7002	30390
Number of reflections	29882
<I/σ(I)>	18.0335
Completeness [%]	99.9	99.16	99.51
Redundancy	7.2	7.06	7.07

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	sitting drop vapor diffusion	5.5	291	25% PEG-3350, 0.2M ammonium sulfate, 0.1M sodium cacodylate, pH 5.5, sitting drop vapor diffusion, temperature 291K
1	sitting drop vapor diffusion	5.5	291	25% PEG-3350, 0.2M ammonium sulfate, 0.1M sodium cacodylate, pH 5.5, sitting drop vapor diffusion, temperature 291K