3U1L

Structure of the mRNA splicing complex component Cwc2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-18
Detector	ADSC QUANTUM 315
Wavelength(s)	0.96393
Spacegroup name	P 21 21 21
Unit cell lengths	44.851, 54.367, 111.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.979 - 1.640
R-factor	0.1721
Rwork	0.171
R-free	0.18930
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.883
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	PHENIX ((phenix.refine: 1.6.3_473))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.700
High resolution limit [Å]	1.640	1.640
Number of reflections	34029
Completeness [%]	99.1	95.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	pH 6.5, 21% PEG 3350, 200mM (NH4)2HCitrate, 100mM Na3Citrate, VAPOR DIFFUSION, HANGING DROP, temperature 291K