3U1C

Anti-parallel dimer of N-terminal 98-aa fragment of smooth muscle tropomyosin alpha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-11-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	76.654, 76.654, 79.693
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.800
R-factor	0.2477
Rwork	0.246
R-free	0.28430
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.140
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXD
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.850
High resolution limit [Å]	1.750	1.750
Rmerge	0.076	0.436
Number of reflections	23590
<I/σ(I)>	24.9	4.59
Completeness [%]	95.9	94.8
Redundancy	17.76	12.69

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	293	0.1 M Sodium acetate, pH 4.0, 40 mM Calcium chloride, 32-35 % 2-METHYL-2,4-PENTANEDIOL, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	4	293	0.1 M Sodium acetate, pH 4.0, 40 mM Calcium chloride, 32-35 % 2-METHYL-2,4-PENTANEDIOL, VAPOR DIFFUSION, HANGING DROP, temperature 293K