3U1B
Crystal structure of the S238R mutant of mycrocine immunity protein (MccF) with AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 43 |
| Unit cell lengths | 118.550, 118.550, 56.619 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.688 - 1.604 |
| R-factor | 0.1651 |
| Rwork | 0.164 |
| R-free | 0.18980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gjz |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.607 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.000 | 1.630 |
| High resolution limit [Å] | 1.604 | 1.604 |
| Rmerge | 0.075 | |
| Number of reflections | 101972 | |
| <I/σ(I)> | 21 | |
| Completeness [%] | 98.9 | 100 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 30% Peg 3350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
