3U0R
Helical repeat structure of apoptosis inhibitor 5 reveals protein-protein interaction modules
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 6C1 |
Synchrotron site | PAL/PLS |
Beamline | 6C1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-29 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.23983, 0.97974, 0.98009, 0.97954, 0.97965 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.423, 88.614, 136.290 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.500 |
R-factor | 0.21696 |
Rwork | 0.216 |
R-free | 0.24223 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.202 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.496 |
Number of reflections | 18250 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 10% PEG 6000, 100mM, bicine pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |