3U0R

Helical repeat structure of apoptosis inhibitor 5 reveals protein-protein interaction modules

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 6C1
Synchrotron site	PAL/PLS
Beamline	6C1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-29
Detector	ADSC QUANTUM 210
Wavelength(s)	1.23983, 0.97974, 0.98009, 0.97954, 0.97965
Spacegroup name	P 21 21 21
Unit cell lengths	46.423, 88.614, 136.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.500
R-factor	0.21696
Rwork	0.216
R-free	0.24223
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.202
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.496
Number of reflections	18250

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	277	10% PEG 6000, 100mM, bicine pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K