3U0O
The crystal structure of selenophosphate synthetase from E. coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-28 |
Detector | MARMOSAIC 300 mm CCD |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 102.347, 102.347, 140.669 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.983 - 2.250 |
R-factor | 0.2239 |
Rwork | 0.222 |
R-free | 0.26390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zod |
RMSD bond length | 0.010 |
RMSD bond angle | 1.522 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.2_865)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.490 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 40980 | |
<I/σ(I)> | 32.7 | 2.8 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 7.4 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 298 | 50 mM Tris-HCl, 50 mM MgCl2, 32% PEG MME 550, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |