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3U0O

The crystal structure of selenophosphate synthetase from E. coli

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 22-ID
Synchrotron siteAPS
Beamline22-ID
Temperature [K]100
Detector technologyCCD
Collection date2005-10-28
DetectorMARMOSAIC 300 mm CCD
Spacegroup nameP 32 2 1
Unit cell lengths102.347, 102.347, 140.669
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution28.983 - 2.250
R-factor0.2239
Rwork0.222
R-free0.26390
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)2zod
RMSD bond length0.010
RMSD bond angle1.522
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwarePHASER
Refinement softwarePHENIX ((phenix.refine: 1.7.2_865))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]30.0002.490
High resolution limit [Å]2.2502.250
Number of reflections40980
<I/σ(I)>32.72.8
Completeness [%]99.899.8
Redundancy7.45.7
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
17.529850 mM Tris-HCl, 50 mM MgCl2, 32% PEG MME 550, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

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