3U0O
The crystal structure of selenophosphate synthetase from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-28 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 102.347, 102.347, 140.669 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.983 - 2.250 |
| R-factor | 0.2239 |
| Rwork | 0.222 |
| R-free | 0.26390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zod |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.522 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.2_865)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.490 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 40980 | |
| <I/σ(I)> | 32.7 | 2.8 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 7.4 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 298 | 50 mM Tris-HCl, 50 mM MgCl2, 32% PEG MME 550, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
