3U0M

Crystal structure of the engineered fluorescent protein mRuby, crystal form 1, pH 8.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-02
Detector	RAYONIX MX225HE
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	31.706, 67.081, 95.001
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.440 - 1.650
R-factor	0.17386
Rwork	0.172
R-free	0.20074
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uis
RMSD bond length	0.027
RMSD bond angle	2.349
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.6.0111)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.660	1.740
High resolution limit [Å]	1.650	1.650
Number of reflections	24632
Completeness [%]	97.9	96.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	0.1 M Tris pH 8.5, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K