3U0L

Crystal structure of the engineered fluorescent protein mRuby, crystal form 1, pH 4.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-02
Detector	RAYONIX MX225HE
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	31.767, 67.439, 95.063
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.030 - 1.250
R-factor	0.12742
Rwork	0.126
R-free	0.16083
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uis
RMSD bond length	0.029
RMSD bond angle	2.528
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.6.0111)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.430	1.320
High resolution limit [Å]	1.250	1.250
Number of reflections	56036
Completeness [%]	97.8	95.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	298	0.1 M Sodium acetate trihydrate pH 4.5, 25% PEG 3,350, VAPOR DIFFUSION, SITTING DROP, temperature 298K