3U01
Crystal structure of onconase double mutant C30A/C75A at 1.12 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2010-01-08 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.288, 38.664, 69.514 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.757 - 1.120 |
| R-factor | 0.13708 |
| Rwork | 0.136 |
| R-free | 0.14956 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1onc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.272 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.757 | 1.150 |
| High resolution limit [Å] | 1.120 | 1.120 |
| Rmerge | 0.344 | |
| Number of reflections | 33823 | |
| <I/σ(I)> | 7.5 | 1.8 |
| Completeness [%] | 97.5 | 83.9 |
| Redundancy | 2.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 30% PEG8000, 0.2 M lithium sulfate, 100 mM sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
