3U00
Crystal structure of wild-type onconase at 1.65 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2011-09-22 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.509, 39.454, 69.985 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.029 - 1.650 |
| R-factor | 0.17661 |
| Rwork | 0.171 |
| R-free | 0.22510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1onc |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.979 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 15.029 |
| High resolution limit [Å] | 1.650 |
| Number of reflections | 11256 |
| <I/σ(I)> | 5.8 |
| Completeness [%] | 93.8 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 20% w/v PEG8000, 0.05 M potassium phosphate monobasic, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
