3TZU
Crystal structure of a glycine cleavage system H protein (GCVH) from Mycobacterium marinum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-09-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97740 |
Spacegroup name | P 31 |
Unit cell lengths | 50.310, 50.310, 165.320 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.272 - 2.300 |
R-factor | 0.187 |
Rwork | 0.184 |
R-free | 0.25200 |
Structure solution method | MR |
Starting model (for MR) | 3ift |
RMSD bond length | 0.012 |
RMSD bond angle | 1.455 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.272 | 2.360 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.072 | 0.482 |
Number of reflections | 20740 | |
<I/σ(I)> | 16.64 | 2.8 |
Completeness [%] | 99.7 | 99.8 |
Redundancy | 5.151 | 4.265 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 290 | Internal tracking number 219005, JCSG screen D04: 0.1 M acetate, pH 4.5, 200 mM lithium sulfate, 30% w/v PEG8000, MymaA.01046.b.A1 PS00874 16.35 mg/mL, VAPOR DIFFUSION, SITTING DROP, temperature 290.0K |