3TZR
Structure of a Riboswitch-like RNA-ligand complex from the Hepatitis C Virus Internal Ribosome Entry Site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 173 |
Detector technology | IMAGE PLATE |
Collection date | 2011-05-31 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 32.054, 33.582, 81.046 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 16.791 - 2.212 |
R-factor | 0.1815 |
Rwork | 0.179 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2nok |
RMSD bond length | 0.006 |
RMSD bond angle | 1.313 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 16.800 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 4664 | |
Completeness [%] | 99.2 | 75.6 |
Redundancy | 6.7 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 10 mM magnesium sulfate, 50 mM sodium cacodylate, 2.0 M ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |