3TZK
Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (FabG)(G92A) from Vibrio cholerae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-06 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 62 |
Unit cell lengths | 63.290, 63.290, 190.431 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.000 - 1.800 |
R-factor | 0.1671 |
Rwork | 0.165 |
R-free | 0.21070 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.405 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 27.000 | 1.830 |
High resolution limit [Å] | 1.800 | 4.880 | 1.800 |
Rmerge | 0.051 | 0.033 | 0.519 |
Number of reflections | 39838 | ||
<I/σ(I)> | 14 | ||
Completeness [%] | 99.9 | 99.4 | 100 |
Redundancy | 9.2 | 9.2 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 9 | 0.1M Tris, 2.4M Ammonium Sulfate, pH 9.0, vapor diffusion |