3TZG
Crystal structure of a porin-like protein (BVU_2266) from Bacteroides vulgatus ATCC 8482 at 2.80 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL14-1 |
Synchrotron site | SSRL |
Beamline | BL14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-24 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.97959,0.96109,0.97938 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 84.663, 84.663, 175.426 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.933 - 2.800 |
R-factor | 0.1987 |
Rwork | 0.197 |
R-free | 0.24100 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.110 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.15) |
Phasing software | SOLVE |
Refinement software | BUSTER-TNT (2.10.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.933 | 29.933 | 2.870 |
High resolution limit [Å] | 2.800 | 12.520 | 2.800 |
Rmerge | 0.048 | 0.742 | |
Total number of observations | 1239 | 8423 | |
Number of reflections | 16458 | ||
<I/σ(I)> | 11.5 | 11.1 | 1 |
Completeness [%] | 99.9 | 92.9 | 100 |
Redundancy | 7 | 5.6 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 277 | 0.1M phosphate-citrate pH 4.2, 40% 2-methyl-2,4-pentanediol, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |