3TZC

Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (FabG)(Y155F) from Vibrio cholerae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-06
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9792
Spacegroup name	P 61
Unit cell lengths	62.353, 62.353, 383.381
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.450
R-factor	0.1902
Rwork	0.188
R-free	0.22720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.536
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.010	50.000	2.490
High resolution limit [Å]	2.450	6.650	2.450
Rmerge	0.108	0.056	0.435
Number of reflections	30294
<I/σ(I)>	7.7
Completeness [%]	98.2	95.2	99.9
Redundancy	3.3	3.3	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5		0.1M Tris, 2.3M Ammonium Sulfate, pH 8.5, vapor diffusion